In vivo recognition of bioactive substances of Polygonum multiflorum for protecting mitochondria against metabolic dysfunction-associated fatty liver disease

Figure 7 Effects of Polygonum multiflorum-derived monomer in liver mitochondria on L02 adipocytes induced by fat emulsion. A: The cell counting kit-8 assay showed that torachrysone had no obvious cytotoxic effect on L02 cells at 12.5 and 25 μmol/L. These two concentrations were selected for in vitro anti-lipid activity detection. L02 cells were induced by fat emulsion for 24 h followed by stimulation with torachrysone (12.5 and 25 μmol/L) or fenofibrate capsules (150 μmol/L) for 24 h. Cells were harvested; B: Levels of total cholesterol were determined using a commercial kit; C: Levels of triglyceride were determined using a commercial kit; D: Levels of alanine transaminase were determined using a commercial kit; E: Levels of aspartate transaminase were determined using a commercial kit; F: Levels of glutathione were determined using a commercial kit; G: Levels of superoxide dismutase were determined using a commercial kit; H: Levels of Na⁺-K⁺-ATPase were determined using a commercial kit; I: Levels of Ca²⁺-Mg²⁺-ATPase were determined using a commercial kit. Data were obtained from 3 independent measurements and are expressed as the mean ± SD. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 vs model group. ALT: Alanine transaminase; AST: Aspartate transaminase; ATPase: ATP synthase; FC: Fenofibrate capsules; GSH: Glutathione; MOD: Model; NC: Normal control; SOD: Superoxide dismutase; TC: Total cholesterol; TG: Triglyceride; TCS: Torachrysone.