Cystic fibrosis transmembrane conductance regulator prevents ischemia/reperfusion induced intestinal apoptosis via inhibiting PI3K/AKT/NF-κB pathway

Figure 2 Cystic fibrosis transmembrane conductance regulator can protect the Caco2 cells from apoptosis induced by the hypoxia/reoxygenation. A: Representative Western blotting and quantification data for cystic fibrosis transmembrane conductance regulator (CFTR) after the hypoxia/reoxygenation (H/R) treatment, ^bP < 0.001 vs control (n = 3); B: Representative Western blotting and quantification data for CFTR after the LV-CFTR transfection, ^bP < 0.001 vs control (n = 3); C: Cell viability was tested using the CCK8 kit after the LV-CFTR transfection, ^bP < 0.001 vs control (n = 3); D: Representative Western blotting and quantification data for cell apoptotic proteins Bcl-2 and Bax after the LV-CFTR transfection. Data are presented as mean ± SD. ^bP < 0.001 vs control (n = 3). CFTR: Cystic fibrosis transmembrane conductance regulator; H/R: Hypoxia/reoxygenation.