Differential DNA methylation analysis of SUMF2, ADAMTS5, and PXDN provides novel insights into colorectal cancer prognosis prediction in Taiwan

Table 2 Characteristics and distribution of methylation status in patients with colorectal cancer (n = 208)

Characteristics	Total	Methylation status
		SUMF2		ADAMTS5		PXDN
		Normal	Tumor	Normal	Tumor	Normal	Tumor
Sex
Male	103 (49.5)	20 (60.6)	27 (55.1)	28 (60.9)	44 (77.2)	35 (76.1)	44 (77.2)
Female	105 (50.5)	17 (45.9)	25 (45.5)	38 (64.4)	49 (73.1)	41 (69.5)	53 (79.1)
χ2 (P value)		0.97 (0.324)	0.62 (0.432)	0.03 (0.866)	0.1 (0.755)	0.28 (0.596)	< 0.01 (0.969)
Age at surgery
mean ± SD	64.3 ± 14.6	64.9 ± 14.2	66.9 ± 15.8	66.4 ± 14.8	67.7 ± 15.5	65.0 ± 14.4	66.2 ± 15.0
< 65	103 (49.5)	17 (51.5)	26 (53.1)	28 (58.3)	43 (72.9)	34 (70.8)	46 (78.0)
≥ 65	105 (50.5)	20 (54.1)	26 (47.3)	38 (66.7)	50 (76.9)	42 (73.7)	51 (78.5)
χ2 (P value)		< 0.01 (1.00)	0.15 (0.694)	0.46 (0.498)	0.1 (0.755)	0.01 (0.915)	< 0.01(1.00)
Stage
I	29 (13.9)	7 (58.3)	7 (50.0)	9 (50.0)	12 (63.2)	12 (66.7)	17 (89.5)
II	77 (37.0)	13 (48.1)	17 (45.9)	21 (56.8)	33 (75.0)	29 (78.4)	39 (88.6)
III	68 (32.7)	13 (65.0)	21 (63.6)	22 (68.8)	31 (75.6)	22 (68.8)	27 (65.9)
IV	34 (16.3)	4 (36.4)	7 (35.0)	14 (77.8)	17 (85.0)	13 (72.2)	14 (70.0)
χ2 (P value)		2.77 (0.429)	4.5 (0.212)	4.06 (0.255)	2.5 (0.476)	1.17 (0.760)	8.69a (0.034)
5-yr recurrence1
No	141 (82.0)	28 (51.9)	35 (49.3)	43 (56.6)	61 (70.9)	54 (71.1)	71 (82.6)
Yes	31 (18.0)	4 (40.0)	9 (60.0)	12 (80.0)	14 (82.4)	11 (73.3)	12 (70.6)
χ2 (P value)		0.12 (0.731)	0.22 (0.639)	1.98 (0.160)	0.45 (0.504)	< 0.01 (1.00)	0.65 (0.421)
5-yr all-cause death
No	168 (80.8)	29 (54.7)	43 (51.8)	50 (61.0)	79 (78.2)	59 (72.0)	77 (76.2)
Yes	40 (19.2)	8 (47.1)	9 (42.9)	16 (69.6)	14 (60.9)	17 (73.9)	20 (87.0)
χ2 (P value)		0.07 (0.786)	0.24 (0.625)	0.26 (0.611)	2.15 (0.142)	< 0.01 (1.00)	0.71 (0.399)
5-yr progression
No	155 (74.5)	28 (56.0)	39 (50.0)	45 (58.4)	73 (76.8)	56 (72.7)	74 (77.9)
Yes	53 (25.5)	9 (45.0)	13 (50.0)	21 (75.0)	20 (69.0)	20 (71.4)	23 (79.3)
χ2 (P value)		0.32 (0.570)	< 0.01(1.00)	1.75 (0.185)	0.38 (0.540)	< 0.01 (1.00)	< 0.01 (1.00)
Lymphovascular invasion1
No	106 (52.5)	20 (48.8)	25 (48.1)	32 (56.1)	46 (73.0)	41 (71.9)	55 (87.3)
Yes	96 (47.5)	16 (57.1)	27 (51.9)	34 (72.3)	47 (78.3)	34 (72.3)	41 (68.3)
χ2 (P value)		0.19 (0.662)	0.04 (0.845)	2.26 (0.133)	0.23 (0.634)	< 0.01 (1.00)	5.44a (0.02)
Histological grade1
Well or moderately	156 (89.7)	27 (48.2)	35 (47.3)	52 (64.2)	67 (74.4)	58 (71.6)	67 (74.4)
Poor or undifferentiated	18 (10.3)	6 (100.0)	9 (64.3)	7 (70.0)	14 (82.4)	9 (90.0)	13 (76.5)
χ2 (P value)		3.94a (0.047)	0.76 (0.382)	< 0.01 (0.99)	0.15 (0.697)	0.75 (0.387)	< 0.01 (1.00)
Lymph node counts1
0-11	34 (18.4)	7 (63.6)	7 (50.0)	12 (80.0)	15 (83.3)	13 (86.7)	16 (88.9)
≥ 12	151 (81.6)	29 (52.7)	40 (50.0)	50 (61.7)	70 (73.7)	58 (71.6)	70 (73.7)
χ2 (P value)		0.07 (0.786)	< 0.01 (1.00)	1.14 (0.287)	0.33 (0.568)	0.81 (0.368)	1.18 (0.278)
Tumor location1
Colon	147 (79.9)	28 (50.0)	38 (50.7)	53 (67.9)	62 (70.5)	60 (76.9)	68 (77.3)
Rectum	37 (20.1)	8 (80.0)	9 (47.4)	9 (50.0)	23 (92.0)	11 (61.1)	18 (72.0)
χ2 (P value)		1.99 (0.158)	< 0.01(1.00)	1.35 (0.245)	3.76 (0.052)	1.17 (0.280)	0.08 (0.780)
Adjuvant chemotherapy1
No	54 (29.3)	10 (58.8)	12 (42.9)	15 (55.6)	25 (73.5)	20 (74.1)	29 (85.3)
Yes	130 (70.7)	26 (53.1)	35 (53.0)	47 (68.1)	60 (75.9)	51 (73.9)	57 (72.2)
χ2 (P value)		0.02 (0.898)	0.46 (0.499)	0.85 (0.358)	0.01 (0.971)	< 0.01(1.00)	1.59 (0.207)

¹The total number of colorectal cancer patients does not correspond because of missing data.

^aP < 0.05. SUMF2: Sulfatase modifying factor 2; ADAMTS5: ADAM metallopeptidase with thrombospondin type 1 motif 5; PXDN: Peroxidasin; SD: Standard deviation.