Differential DNA methylation analysis of SUMF2, ADAMTS5, and PXDN provides novel insights into colorectal cancer prognosis prediction in Taiwan

doi:10.3748/wjg.v28.i8.825

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Feb 28, 2022 (publication date) through Jul 7, 2024

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World Journal of Gastroenterology

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1007-9327

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Retrospective Cohort Study

World J Gastroenterol. Feb 28, 2022; 28(8): 825-839
Published online Feb 28, 2022. doi: 10.3748/wjg.v28.i8.825

Table 1 Primer sequences, annealing temperature and product size for MS-PCR and EpiTYPER DNA methylation analysis of target genes

Genes		Forward primer (5’ → 3’)	Annealing temperature (°C)	Product size (bp)
SUMF2	M	F:TTTGATTATGGTCGGTTTTGC	59.4	191
	M	R:GACTACTTACAACTCCCCTAACGAC	59.4	191
	U	F:TTTTTTGATTATGGTTGGTTTTGTG	60.6	198
	U	R:CCCAACTACTTACAACTCCCCTAACA	60.6	198
	Q	F:TTTGTTATAGAGGGATGGGAGATAG aggaagagag	60	232
	Q	R:CAAAATAAACAACACTCCAAATTCA cagtaatacgactcactatagggagaaggct	60	232
ADAMTS5	M	F:GTTATTGTCGTGGAGCGTTAGC	59.4	170
	M	R:CCTACCTCCCGTACTTCCCG	59.4	170
	U	F:TTATTGTTGTGGAGTGTTAGTGTTT	59.4	169
	U	R:CCTACCTCCCATACTTCCCACAT	59.4	169
	Q	F:aggaagagagTTGAAATTGTTATTGTAGGATGGTATG	61.3	245
	Q	R:cagtaatacgactcactatagggagaaggctAATTAAAACAAAAATACAAAAAAACAACC	61.3	245
PXDN	M	F:TATGCGGGACGAGAACGAGA	61.6	137
	M	R:ACTTAAACAACTCCGTAACAATACGAT	61.6	137
	U	F:GTGTATGTGGGATGAGAATGAGAG	60.4	142
	U	R:CAACTTAAACAACTCCATAACAATACAA	60.4	142

MS-PCR: Methylation-specific polymerase chain reaction; SUMF2: Sulfatase modifying factor 2; ADAMTS5: ADAM metallopeptidase with thrombospondin type 1 motif 5; PXDN: Peroxidasin; M: Methylation; U: Unmethylation; Q: Quantitative analysis.

Citation: Su JQ, Lai PY, Hu PH, Hu JM, Chang PK, Chen CY, Wu JJ, Lin YJ, Sun CA, Yang T, Hsu CH, Lin HC, Chou YC. Differential DNA methylation analysis of SUMF2, ADAMTS5, and PXDN provides novel insights into colorectal cancer prognosis prediction in Taiwan. World J Gastroenterol 2022; 28(8): 825-839
URL: https://www.wjgnet.com/1007-9327/full/v28/i8/825.htm
DOI: https://dx.doi.org/10.3748/wjg.v28.i8.825