Bladder-colon chronic cross-sensitization involves neuro-glial pathways in male mice

Figure 6 Effect of CP 99994 injection at the central level on visceral sensitivity and the expression of phosphorylated MAPK-p38. A: Changes in intracolonic pressure in response to isobaric colorectal distensions (15, 30, 45 and 60 mmHg) after intracerebroventricular (ICV) injections of saline (NaCl 0.9%) or CP 99994 (7.5 mg/kg) prior to intravesical (IV) injection of saline or acetic acid 0.75% in mice at day 7, with details of the comparison at 60 mmHg of distension on the right. n = 8 mice per group; B: Expression of phosphorylated MAPK-p38 (P-p38) at the L6-S1 level of the spinal cord, with quantitative analysis (ratio to the reference protein GAPDH) on the right. Samples were run on separate gels and compiled for the figure. n = 5-8 mice per group. ^aP < 0.05, ^bP < 0.01 and ^cP < 0.001 (compared to the saline ICV + saline IV group); ^dP < 0.05 and ^eP < 0.001 (compared to the CP 99994 ICV + acetic acid IV group). Variability in the results is represented by the standard error of the mean (area under the curve ± standard error of the mean). AUC: Area under the curve.