Bladder-colon chronic cross-sensitization involves neuro-glial pathways in male mice

Figure 3 Quantification of c-Fos immunoreactive cells. Quantification of c-Fos immunoreactive (IR) cells in lamina I and II of the dorsal horn per slice of the L6-S1 spinal cord on day 7 after intravesical injection of saline (NaCl 0.9%) or acetic acid 0.75%, with or without prior colorectal distensions (CRDs) at 45 mmHg for 120 min (n = 6-7 mice per group). ^aP < 0.05 and ^bP < 0.0001 (compared to mice treated with saline and no prior CRD); ^cP < 0.001 (compared to mice treated with saline and prior CRD); ^dP < 0.01 (compared to mice treated with acetic acid and no prior CRD). Results are expressed as the mean ± standard error of the mean.