Interleukin-34 deficiency aggravates development of colitis and colitis-associated cancer in mice

Figure 6 Interleukin-34 deficiency delays the mucosal healing in murine model induced by dextran sodium sulfate. A: Establishing healing model chart. The Interleukin-34 (IL-34)^-/- and WT mice were fed with 3% dextran sodium sulfate (DSS) for 5 d and switched to normal drinking water for the following 5 d. The mice in both groups were killed on day 8 or 10 (n = 4 or 5 per group); B and C: Body weight (B) and clinical score (C) were determined daily in IL-34^-/- and WT mice until day 10 (n = 4 or 5 per group); D and E: The colon length was measured in IL-34^-/- and WT mice on day 8 and 10, respectively (n = 4 or 5 per group); F and G: Representative microscopic pictures (F) and semiquantitative histological scores (G) of IL-34^-/- and WT mice on day 8 and 10, respectively (n = 4 or 5 per group); H and I: Representative photomicrographs of Ki-67 immunohistochemical staining (H) and percentage of Ki-67-positive per crypt (I) in IL-34^-/- and WT mice on day 8 and 10, respectively. DSS: Dextran sodium sulfate; IL-34: Interleukin-34; WT: Wild type. Scale bars = 200 μm. Data depict the mean ± SD. ^aP < 0.05, ^bP < 0.01, ^cP < 0.005.