3,6-dichlorobenzo[b]thiophene-2-carboxylic acid alleviates ulcerative colitis by suppressing mammalian target of rapamycin complex 1 activation and regulating intestinal microbiota

Figure 5 3,6-dichlorobenzo[b]thiophene-2-carboxylic acid reduced cyclooxygenase-2 expression. A and B: The level of cyclooxygenase-2 (COX-2) in colon tissues was detected by Western blotting; C: The mRNA level of COX-2 in colon tissues was detected by real-time polymerase chain reaction. ^fP < 0.001 vs the dextran sodium sulfate group; ^cP < 0.001 vs the control group. The data are shown as the mean ± SD (n = 4). COX-2: Cyclooxygenase-2; DSS: Dextran sodium sulfate; SASP: Salazosulfapyridine; BT2: 3,6-dichlorobenzo[b]thiophene-2-carboxylic acid.