3,6-dichlorobenzo[b]thiophene-2-carboxylic acid alleviates ulcerative colitis by suppressing mammalian target of rapamycin complex 1 activation and regulating intestinal microbiota

Figure 4 3,6-dichlorobenzo[b]thiophene-2-carboxylic acid suppressed the activity of the mammalian target of rapamycin complex 1 activation. A-D: The total and phosphorylated proteins of S6, eukaryotic translation initiation factor 4E binding protein 1 (4EBP1) and mammalian target of rapamycin were measured by Western blot analysis; E: Immunohistochemical analysis of the expression of phosphorylated S6 and phosphorylated 4EBP1 in colon tissues (200 × magnification). ^dP < 0.05, ^eP < 0.01, and ^fP < 0.001 vs the dextran sodium sulfate group; ^bP < 0.01 and ^cP < 0.001 vs the control group. The data are shown as the mean ± SD (n = 3-5). mTOR: Mammalian target of rapamycin; S6: Ribosomal protein S6; 4EBP1: Eukaryotic translation initiation factor 4E binding protein 1; DSS: Dextran sodium sulfate; SASP: Salazosulfapyridine; BT2: 3,6-dichlorobenzo[b]thiophene-2-carboxylic acid.