Hypoxia inducible factor 1α promotes interleukin-1 receptor antagonist expression during hepatic ischemia-reperfusion injury

Figure 7 Ischemic preconditioning protects the liver by promoting interleukin-1 receptor antagonist expression via hypoxia-inducible factor-1α. A: Serum alanine transaminase (ALT) levels in mice undergoing ischemia-reperfusion injury (IRI) after different ischemic preconditioning (IP) cycles; B: Serum aspartate transaminase (AST) levels in mice undergoing IRI after different IP cycles; C: Protein levels of interleukin-1 receptor antagonist (IL-1ra) in the liver after different IP cycles; D: Statistical analysis of protein levels of IL-1ra after different IP cycles; E: Protein content of IL-1ra in serum after different IP cycles; F: Protein levels of IL-1ra in the liver with shIl1ra injection after three IP cycles; G: Statistical analysis of protein levels of IL-1ra with shIl1ra injection after three IP cycles; H: Protein content of IL-1ra in serum with shIl1ra injection after three IP cycles; I: Serum ALT levels in mice with shIl1ra injection after three IP cycles; J: Serum AST levels in mice with shIl1ra injection after three IP cycles; K: Protein levels of hypoxia-inducible factor (HIF)-1α and IL-1ra in the liver after different IP cycles; L: Statistical analysis of protein levels of HIF-1α after different IP cycles; M: Statistical analysis of protein levels of IL-1ra after different IP cycles; N: Protein levels of HIF-1α and IL-1ra in the liver with 2-MeOE2 treatment after different IP cycles; O: Statistical analysis of protein levels of HIF-1α with 2-MeOE2 treatment after different IP cycles; P: Statistical analysis of protein levels of IL-1ra with 2-MeOE2 treatment after different IP cycles; Indicated P values were calculated using two-tailed unpaired Student’s t-tests. Data are presented as the mean ± SEM. Data are representative of five independent experiments. ^nsP > 0.05, ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. IP: Ischemic preconditioning; AST: Aspartate transaminase; ALT: Alanine transaminase; IL-1ra: Interleukin-1 receptor antagonist; HIF: Hypoxia-inducible factor.