Hypoxia inducible factor 1α promotes interleukin-1 receptor antagonist expression during hepatic ischemia-reperfusion injury

Figure 5 Hypoxia-inducible factor-1α promotes the expression of interleukin-1 receptor antagonist during ischemia or hypoxia. A: Protein levels of hypoxia-inducible factor (HIF)-1α in the liver upon ischemia; B: Statistical analysis of protein levels of HIF-1α in the liver upon ischemia; C: Protein levels of HIF-1α in AML12 cells upon hypoxia; D: Statistical analysis of protein levels of HIF-1α in AML12 cells upon hypoxia; E: Immunofluorescence staining for HIF-1α in AML12 cells upon hypoxia for 1.5 h. Scale bars, 10 μm; F: Relative mRNA levels of interleukin-1 receptor antagonist (Il1ra) in AML12 cells treated with 2-MeOE2 (100 μΜ) and DMOG (100 μΜ) upon hypoxia; G: Protein levels of HIF-1α and IL-1ra in AML12 cells treated with 2-MeOE2 and DMOG upon hypoxia; H: Statistical analysis of protein levels of HIF-1α in AML12 cells treated with 2-MeOE2 and DMOG upon hypoxia; I: Statistical analysis of protein levels of IL-1ra in AML12 cells treated with 2-MeOE2 and DMOG upon hypoxia; J: Protein content of IL-1ra in culture medium of in AML12 cells treated with 2-MeOE2 and DMOG upon hypoxia. Indicated P-values were calculated using two-tailed unpaired Student’s t-tests. Data are presented as the mean ± SEM. Dots depict individual samples. ^nsP > 0.05, ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. Hypo: Hypoxia; IS: Ischemia; HIF: Hypoxia-inducible factor; IL-1ra: Interleukin-1 receptor antagonist; IgG: Immunoglobulin G.