Hypoxia inducible factor 1α promotes interleukin-1 receptor antagonist expression during hepatic ischemia-reperfusion injury

Figure 4 Interleukin-1 receptor antagonist expression is elevated during ischemia or hypoxia. A: Relative mRNA levels of interleukin-1 receptor antagonist (Il1ra) in the liver upon ischemia; B: Protein levels of IL-1ra in the liver upon ischemia; C: Statistical analysis of protein levels of IL-1ra upon ischemia; D: Protein content of IL-1ra in serum upon ischemia; E: Relative mRNA levels of Il1ra in AML12 cells upon hypoxia; F: Protein levels of IL-1ra in AML12 cells upon hypoxia; G: Statistical analysis of protein levels of IL-1Ra in AML12 cells upon hypoxia; H: Protein content of IL-1ra in culture medium upon ischemia. Indicated P values were calculated using two-tailed unpaired Student’s t-tests. Data are presented as the mean ± SEM. Data are representative of five independent experiments. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. Hypo: Hypoxia; IS: Ischemia; IL-1ra: Interleukin-1 receptor antagonist.