Hypoxia inducible factor 1α promotes interleukin-1 receptor antagonist expression during hepatic ischemia-reperfusion injury

Figure 2 Interleukin-1 receptor antagonist expression is elevated after hepatic ischemia-reperfusion injury in mice. A: Relative mRNA levels of interleukin-1 receptor antagonist (Il1ra) in the liver upon ischemia-reperfusion injury (IRI); B: Protein levels of IL-1ra in the liver upon IRI; C: Statistical analysis of protein levels of IL-1Ra upon IRI; D: Protein content of IL-1ra in serum upon IRI; E. Representative images of immunohistochemical staining for IL-1ra in the liver upon IRI. Scale bars, 100 μm; F: Statistical analysis of immunohistochemical scores. Indicated P values were calculated using a two-tailed unpaired Student’s t-test. Data are presented as the mean ± SEM. Data are representative of five independent experiments. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. IS: Ischemia; IR: Ischemia-reperfusion; IL-1ra: Interleukin-1 receptor antagonist.