Lentivirus-mediated short hairpin RNA interference of CENPK inhibits growth of colorectal cancer cells with overexpression of Cullin 4A

doi:10.3748/wjg.v28.i37.5420

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Oct 7, 2022; 28(37): 5420-5443
Published online Oct 7, 2022. doi: 10.3748/wjg.v28.i37.5420

Table 3 Lentiviral-infected HCT 116 cells

Virus number	Experimental group	Virus titer	Virus infection dose
psc3741	NC (KD)	2.0E + 08 TU/mL	10.00 μL
LVpGCSIL-004PSC45249-1	KD	3.0E + 08 TU/mL	6.67 μL
KL9688-21	NC (OE)	4.0E + 8 TU/mL	5.00 μL
LVKL6733-2	OE	2.0E + 08 TU/mL	10.00 μL
LVpGCSIL-004PSC45249-1+ KL9688-21	KD+NC (OE)	3.0E + 08 + 2E + 8 TU/mL	3.33 + 5.00 μL
LVpGCSIL-004PSC45249-1+ LVKL6733-2	KD+OE	3.0E + 08 + 2E + 8 TU/mL	3.33 + 5.00 μL

NC: HCT 116 cells infected with centromere protein K negative control virus; KD: HCT 116 cells with centromere protein K gene short hairpin RNA virus infection; OE: HCT 116 cells virally infected with Cullin 4A.

Citation: Li X, Han YR, Xuefeng X, Ma YX, Xing GS, Yang ZW, Zhang Z, Shi L, Wu XL. Lentivirus-mediated short hairpin RNA interference of CENPK inhibits growth of colorectal cancer cells with overexpression of Cullin 4A. World J Gastroenterol 2022; 28(37): 5420-5443
URL: https://www.wjgnet.com/1007-9327/full/v28/i37/5420.htm
DOI: https://dx.doi.org/10.3748/wjg.v28.i37.5420