Long noncoding RNA negative regulator of antiviral response contributes to pancreatic ductal adenocarcinoma progression via targeting miR-299-3p

Figure 5 Negative regulator of antiviral response acted as a molecular sponge for miR-299-3p. A: Subcellular fractionation assay was used to determine the subcellular localization of NRAV; B: A complementary binding site of NRAV in miR-299-3p was predicted via starBase v3.0 online database; C: RNA immunoprecipitation assays were performed to validate whether NRAV could interact with miR-299-3p in MiaPaca-2 and BxPC-3 cells; D: Luciferase reporter assays were carried out to verify the direct binding of NRAV and miR-299-3p in MiaPaca-2 and BxPC-3 cells; E: Relative expression of miR-299-3p in AsPC-1 and PANC-1 cells following knockdown of NRAV; F: Spearman’s correlation analysis between NRAV and miR-299-3p expression in PAAD by the starBase v3.0 project. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001. NRAV: Negative regulator of antiviral response; MUT: Mutant; NC: Negative control; WT: Wild-type.