Ji-Chuan decoction ameliorates slow transit constipation via regulation of intestinal glial cell apoptosis

Figure 5 Integrative analysis and experimental validation of improved network pharmacology and metabolites. A: Integration analysis between the modified network pharmacology and metabolites. The ordinate stands for pathways, the primary abscissa stands for minus false discovery rates, and the secondary abscissa stands for impact; B and C: The relative expression of Caspase3+ Glial fibrillary acidic protein was assessed by immunofluorescent staining. GFAP: Glial fibrillary acidic protein; HC: Healthy control; STC: Slow transit constipation; JCD: Ji-Chuan decoction; JCDL: Low-dose Ji-Chuan decoction treatment; JCDM: Middle-dose Ji-Chuan decoction treatment; JCDH: High-dose Ji-Chuan decoction treatment; MSP: Mosapride treatment; FDR: False discovery rates; KEGG: Kyoto Encyclopedia of Genes and Genomes; Th: Thyroid hormone; EGFR: Epidermal growth factor receptor; MAPK: Mitogen-activated protein kinase; IL: Interleukin; TNF: Tumor necrosis factor; HIF: Hypoxia-inducible factor. Data are expressed as the mean ± SD. Compared with the healthy control group: ^aP < 0.05, ^bP < 0.01. Compared with the slow transit constipation group: ^cP < 0.05, ^dP < 0.01.