Long noncoding RNA ZNFX1-AS1 promotes the invasion and proliferation of gastric cancer cells by regulating LIN28 and CAPR1N1

Figure 2 ZNFX1-AS1 is a potential diagnostic and prognostic biomarker. A: Scatter plot of long noncoding RNA (lncRNA) relative expression levels in the plasma of healthy control subjects (normal; n = 60), gastritis/peptic ulcer patients (Gs/PU patients; n = 60), gastric stromal tumor patients (GIST; n = 60), preoperative patients (pre-opt; n = 60), and postoperative patients (post-opt; n = 60), as assessed by real-time polymerase chain reaction (PCR); B: Scatter plot of lncRNA relative expression levels in the plasma of healthy control subjects (normal; n = 60), early-stage patients (TNM I + II; n = 20), and advanced-stage patients (TNM III + IV; n = 40), as assessed by real-time PCR; C: The endpoints indicate the relative expression levels of lncRNA ZNFX1-AS1 (ZFAS1) in preoperative (pre-opt) and postoperative (post-opt) patient plasma, while the lines connecting the pairs of endpoints indicate the trends in the relative expression levels in the matched preoperative and postoperative patient plasma; D: The linear correlations between the relative expression of lncRNAs in plasma and infrequent clonal complexes were analyzed. P values (^aP < 0.05; ^bP < 0.01; ^cP < 0.001) were determined using the t-test; E: Receiver operating characteristic curves showing the area under the curve values of plasma ZFAS1 and traditional serum biomarkers; F: Progression-free survival times indicating the potential prognostic value of ZFAS1 in gastric cancer patients; G: Overall survival times indicating the potential prognostic value of ZFAS1 in gastric cancer patients. Gs/PU: Gastritis/peptic ulcer patients; GIST: Gastric stromal tumor; AUC: Area under the curve; CEA: Carcinoembryonic antigen; AFP: Alpha fetoprotein; CA-199: Carbohydrate antigen 199; OS: Overall survival; PFS: Progression-free survival.