Sirolimus increases the anti-cancer effect of Huai Er by regulating hypoxia inducible factor-1α-mediated glycolysis in hepatocellular carcinoma

Figure 5 Effects of sirolimus & Huai Er on expression of target genes under under normoxic and hypoxic conditions. A: The mRNA levels of Akt, p27, PTEN, FOXP3, and VEGF in cells treated with sirolimus (SRL) under normoxic condiction at 24 h and 48 h; B: The mRNA levels of Akt, mTOR, FOXP3, and VEGF in cells treated with Huai Er under normoxic condiction at 24 h and 48 h; C: The mRNA level of Akt, mTOR, VEGF, HIF-1α, LDHA, GLUT-1, FOXP3, p27, and PTEN in cells treated with SRL and Huai Er under hypoxia at 24 h and 48 h. ^aP < 0.05 vs control; ^bP < 0.05 vs control; ^cP < 0.01 vs hypoxia; ^dP < 0.001 vs hypoxia. PTEN: Phosphatase and tensin homolog deleted on chromosome ten; FoxP3: Forkhead box P3; VEGF: Vascular endothlial growth factor; mTOR: Mammalian target of rapamycin; HIF-1α: Hypoxia inducible factor-1α; LDHA: Lactate dehydrogenase A; GLUT-1: Glucose transporter 1.