Anti-tumour activity and toxicological studies of combination treatment of Orthosiphon stamineus and gemcitabine on pancreatic xenograft model

Table 2 Effect of C5EOSEW5050ESA and gemcitabine alone or in combination on mouse body weight

No.	Group	Days											P value
		0	2	5	8	11	14	17	20	23	26	28
1	Untreated	24.2 ± 0.8	24.5 ± 1.0	24.7 ± 0.6	24.7 ± 0.9	24.6 ± 1.1	24.8 ± 0.9	25.0 ± 0.3	24.8 ± 0.6	24.6 ± 0.7	24.5 ± 1.1	24.5 ± 0.9	-
2	Gemcitabine (10 mg/kg per 3 d)	24.2 ± 0.7	24.3 ± 1.5	24.1 ± 1.2	23.9 ± 1.7	24.1 ± 1.3	24.0 ± 1.3	24.2 ± 0.5	24.5 ± 0.9	24.6 0 ± .5	24.3 ± 0.7	24.0 ± 0.9	NS
3	O.s (200 mg/kg per day)	23.6 ± 0.6	23.9 ± 0.7	24.9 ± 0.9	23.9 ± 1.1	24.1 ± 1.2	23.8 ± 1.1	24.0 ± 0.7	23.9 ± 9.0	23.8 ± 1.2	24.0 ± 1.5	23.8 ± 0.5	NS
4	O.s (200 mg/kg per day) and gemcitabine (10 mg/kg per 3 d) combination	24.0 ± 0.8	24.0 ± 1.2	23.9 ± 1.0	24.0 ± 1.1	23.4 ± 1.3	23.9 ± 1.5	24.1 ± 0.7	24.2 ± 0.7	23.9 ± 1.3	23.7 ± 1.1	23.5 ± 1.1	NS
5	O.s (400 mg/kg per day)	24.2 ± 0.7	24.4 ± 0.8	24.3 ± 1.0	24.5 ± 1.3	24.1 ± 1.0	24.3 ± 1.0	24.2 ± 1.0	24.4 ± 1.3	24.1 ± 1.4	24.0 ± 1.5	23.8 ± 1.2	NS
6	O.s (400 mg/kg per day) and gemcitabine (10 mg/kg per 3 d) combination	23.9 ± 0.7	24.0 ± 0.2	23.4 ± 0.7	23.4 ± 0.7	23.4 ± 0.5	23.4 ± 0.4	23.3 ± 0.5	23.5 ± 0.5	23.3 ± 0.8	23.3 ± 0.5	23.3 ± 0.3	NS

Each group contains 6 mice. The results showed no significant difference in the reduction of body weight of mice compared to the untreated group. Data are represented as mean ± SD. (NS: Not significant compared to the untreated group, P > 0.05). No statistical difference was also observed among all treatment groups using Two-way ANOVA and Tukey’s honest significant difference posthoc test. O.s: Orthosiphon stamineus.