Duodenal-jejunal bypass increases intraduodenal bile acids and upregulates duodenal SIRT1 expression in high-fat diet and streptozotocin-induced diabetic rats

Figure 3 Duodenal-jejunal bypass increased SIRT1 expression and promoted phosphorylation of AMPK in the duodenal mucosa. A: Western blot images of SIRT1, phosphorylated AMPK (p-AMPK), and total AMPK (t-AMPK) in the descending duodenum; B and C: The relative intensity of SIRT1 and p-AMPK/t-AMPK in the descending duodenum; D: Western blot images of SIRT1, p-AMPK, and t-AMPK in the horizontal duodenum; E and F: The relative intensity of SIRT1 and p-AMPK/t-AMPK in the horizontal duodenum. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 duodenal-jejunal bypass vs sham group. p-AMPK: Phosphorylated AMPK; t-AMPK: Total AMPK; DJB: Duodenal-jejunal bypass.