Role of one-step nucleic acid amplification in colorectal cancer lymph node metastases detection

Table 3 Differences in lymph node processing methods and upstage rates of previous reports

Ref.	Subject (patients)	OSNA method	Harvested LN, n	Harvested LN, median	Dividing method of LN	Pathological staining	Measured LN by OSNA, n	Measured LN by OSNA, median	Upstage rate (pStage I and II)
Yamamoto et al[16]	Stage 0, I (85)	cOSNA	434	N/Aa	Fourb; 4 mm over diameter of LN	HE and IHC	385	4.5	16.5% (2/16)
Güller et al[12]	Stage I, II, III (22)	cOSNA	313	30 (16–60)	Fourb; 3 mm over diameter of LN	HE and IHC	56	13 (6–24)	15.3% (2/13)
Croner et al[14]	Stage I, II (103)	cOSNA	N/Aa	N/Aa	Centerc; 6 mm over halfd; 4 mm to 6 mm diameter of LN	HE	1594	14 (1–46)	25.2% (26/103)
Vogelaar et al[15]	Stage I, II (128)	cOSNA	N/Aa	N/Aa	Fourb; 10 mm over halfd; 10 or less than 10 mm diameter of LN	HE and IHC	317	Mean 15.3 (4–40)	20.2% (20/90)
Yamamoto et al[17]	Stage I, II, III (204)	cOSNA	4324	19 (3–25)	Halfd	HE	1925	8 (2–25)	17.6% (13/74)
Aldecoa et al[133]	Stage I, II (149)	cOSNA	2483	15	Centerc	HE	1940	12	51% (76/149)
Rakislova et al[125]	Stage I, II, III (188)	cOSNA, pOSNA	cOSNA 1828, pOSNA 1992	cOSNA 17 (13-22), pOSNA 20.5 (17-27)	Centerc	HE	cOSNA 1757, pOSNA 1449	cOSNA 13 (10-18), pOSNA 18 (13-25)	cOSNA 55.4% (51/92), pOSNA 20.7% (16/77)
Brito et al[126]	Stage I, II (59)	cOSNA	1046	13 (9-19)	Centerc; 5 mm over halfd; 4 or less diameter of LN	HE	753	12 (7-16)	28.8% (17/59)
Itabashi et al[18]	Stage I, II, III (195)	cOSNA	Not shown	19 (1-75)	Halfd; 4 mm over diameter of LN	HE	Not shown	8 (2-25)	15.7% (11/70) in stage II patients
Tani et al[33]	Stage II, IIIA (92)	pOSNA	2236	24.3 (5–66)	Halfd; 4 mm over diameter of LN	HE	636	6.9 (1–35)	9.1% (6/66)

^aNot applicable.

^bFour section method: dividing lymph nodes into four equal sections and sending two of these sections (50%) for pathology and one-step nucleic acid amplification (OSNA) measurement.

^cCenter-cut method: 1 mm from the center of lymph nodes are sent for pathological examination and the rest are used for OSNA.

^dHalf-division method divides lymph nodes in half and sends each 50% portion for pathology and OSNA.

HE: Hematoxylin and eosin; IHC: Immunohistochemistry; LN: Lymph node; OSNA: One-step nucleic acid amplification; pOSNA: Pooled one-step nucleic acid amplification.