Role of one-step nucleic acid amplification in colorectal cancer lymph node metastases detection

Table 2 A comparison of the diagnostic accuracy of the one-step nucleic acid amplification assay in colorectal cancer patients

Ref.	Pathological evaluation	IHC	LN number inspected by OSNA, mean	Sensitivity, %	Specificity, %	Concordance, %	PPV, %	NPV, %
Croner et al[11]	Multi-slice	CK19	1.0	92.5	96.5	93.6	88.1	97.9
Yamamoto et al[16]	Multi-slice	CK19	4.5	95.2	97.7	97.1	91.9	98.7
Güller et al[12]	Multi-slice	CK19	14.2	94.5	97.6	97.1	89.7	98.8
Yamamoto et al[17]	Single-slice	None	9.4	86.2	96.5	95.7	66.5	98.8
Colling et al[129]	Single-slice	None	4.3	92.9	97.1	96.3	86.7	98.5
Yeung et al[130]	Single-slice	None	4.9	100	98.4	98.7	94.1	100
Esposito et al[127]	Multi-slice	None	1.5	69.2	100	88.2	100	84.0
Rakislova et al[125]	Not shown	None	20.5 (pOSNA)	88.9	79.2	80.2	33.3	98.4
Vogelaar et al[15]	Multi-slice	Anti pan-cytokeratin	15.3	51.6	84.1	67.7	76.7	63.1
Miyake et al[193]	Single-slice	CEA	11	100	86	88	57	100
Marhic et al[184]	Not shown	None	Not shown	50	100	70.6	100	58
Numata et al[188] (predictive value for pathological LatLNM testing OSNA-MesLNM)	Not shown	None	17	100	55	61.8	28	100
Tani et al[33]	Not shown	CK19	6.9 (pOSNA)	84.6	90.9	89.1%	78.6	93.7

CEA: Carcinoembryonic antigen; CK19: Cytokeratin 19; IHC: Immunohistochemistry; LatLNM: Lateral lymph node metastasis; MesLNM: Mesorectal lymph node metastasis; NPV: Negative predictive value; OSNA: One-step nucleic acid amplification; pOSNA: Pooled one-step nucleic acid amplification; PPV: Positive predictive value.