Role of one-step nucleic acid amplification in colorectal cancer lymph node metastases detection

Table 1 Characteristics of one-step nucleic acid amplification studies in colorectal cancer patients

Ref.	Nation	Type of study	Patients number (sample number)	Tumor type	Purpose of the OSNA analysis
Croner et al[11], 2010	Germany	Prospective study	184 (184)	Colorectal	Diagnosis of LN metastasis
Yamamoto et al[16], 2011	Japan	Prospective multicenter study	85 (385)	Colorectal	Diagnosis of LN metastasis
Güller et al[12], 2012	Switzerland	Prospective study	22 (313)	Colon	Diagnosis of LN metastasis
Yamamoto et al[13], 2013	Japan	Not shown	30 (66)	Colorectal	Identification of CK19
Croner et al[14], 2014	Germany	Prospective multicenter study	103 (1594)	Colon	Pathologically nodenegative CC
Vogelaar et al[15], 2014	Switzerland	Prospective multicenter study	128 (325)	Colon	Diagnosis of SLN metastasis
Yamamoto et al[17], 2016	Japan	Prospective multicenter study	204 (1925)	Colorectal	Diagnosis of LN metastasis
Aldecoa et al[133], 2016	Spain	Prospective multicenter study	149 (1940)	Colorectal	Correlation between TTL and tumor’s characteristics
Rakislova et al[125], 2017	Spain	Observational study	188 (3206)	Colon	Diagnosis of pooled LN metastasis
Miyake et al[193], 2017	Japan	Prospective study	25 (306)	Rectum	Indication of LPLN dissection
Marhic et al[184], 2017	France	Prospective study	17	Colon	Diagnosis of SLN metastasis
Colling et al[129], 2-17	United Kingdom	Prospective study	19 (82)	Colorectal	Diagnosis of LN metastasis
Aldecoa et al[124], 2017	Spain	Prospective study	71 (936)	Colon	OSNA with endoscopic tattooing
Yeung et al[130], 2018	United Kingdom	Prospective study	16 (78)	Colorectal	OSNA with ICG detection
Brito et al[126], 2018	Portugal	Prospective multicenter study	59 (753)	Colon	Pathologically node negative CRC
Esposito et al[127], 2019	Italy	Prospective study	34 (51)	Colorectal	Diagnosis of SLN metastasis
Diaz-Mercedes et al[109], 2019	Spain	Prospective study	17 (980)	Colorectal	Budget impact analysis
Itabashi et al[18], 2020	Japan	Prospective multicenter study	195	Colorectal	Prognostic value of the OSNA assay for pStage IICRC patients
Archilla et al[145], 2021	Spain	Retrospective multicenter study	342 (5931)	Colorectal	Correlation between the TTL with patient outcome
Weixler et al[147], 2021	Netherlands, Germany, Switzerland	Retrospective multicenter study	87	Colon	Prognostic value of OSNA
Tani et al[33], 2021	Japan	Prospective multicenter study	92	Colon	Diagnosis of pooled LN metastasis
Numata et al[188], 2021	Japan	Prospective study	34	Rectum	Indication of LPLN dissection

CRC: Colorectal cancer; ICG: Indocyanine green; LPLN: Lateral pelvic lymph node; OSNA: One-step nucleic acid amplification; SLN: Sentinel lymph node; TTL: Total tumor load.