Involvement of toll-like receptor 5 in mouse model of colonic hypersensitivity induced by neonatal maternal separation

Figure 2 Neonatal maternal separation induces colonic hypersensitivity and increases intestinal permeability in mice. A: Intracolonic pressure variation (IPV) in response to colorectal distension in non-handled (NH), neonatal maternal separated non-sensitized (NMS NS) and neonatal maternal separated sensitized (NMS S) mice; B: Area under the curve (AUC) of the IPV relative to colorectal distension for each NH, NMS NS and NMS S mouse; C: FITC-dextran 4 kDa plasmatic concentrations, 3 h after oral gavage with 15 mg of FITC-dextran of NH, NMS NS and NMS S mice. Values are expressed as a percentage of FITC-dextran per mL of plasma in comparison to the NH group mean. NH: n = 6; NMS NS: n = 6; NMS S: n = 8. ^aP < 0.05 and ^bP < 0.01 vs NH group; and ^dP < 0.05, ^eP < 0.01 and ^fP < 0.001 vs NMS NS group. For IPV to CRD test, dots represent means and error bars represent SEM. For AUC and FITC-dextran, each dot represents one mouse and red lines represent means.