High-fat diet aggravates colitis via mesenteric adipose tissue derived exosome metastasis-associated lung adenocarcinoma transcript 1

Figure 4 Associations between metastasis-associated lung adenocarcinoma transcript 1 and miR-15a-5p/activating transcription factor 6. A: Prediction of miR-15a-5p binding site on the 3’ untranslated region of activating transcription factor 6 (ATF6); B: Western blotting to detect ATF6 protein; C: Dual luciferase report of miR-15a-5p and ATF6; D: RNA pull down analysis of miR-15a-5p and metastasis-associated lung adenocarcinoma transcript 1. ATF6: Activating transcription factor 6; TNBS: 2,4,6-trinitrobe-nzenesulfonic acid; HFD: High-fat diet; ND: Normal diet; 3’ UTR: 3’ untranslated region; WT: Wild type; Mut: Mutant. ^aP < 0.05, ^bP < 0.01.