Comprehensive evaluation of microRNA as a biomarker for the diagnosis of hepatocellular carcinoma

Table 3 Univariate analysis of real-time quantitative PCR results for comparison

miRNA	Wilcoxon Q-value	AUC	Specificity at 90% sensitivity
All HCC vs CLD
hsa-miR-21-5p	0.000003a	0.71	0.24
hsa-miR-320d	0.010872b	0.63	0.25
hsa-miR-423-5p	0.046214b	0.61	0.26
hsa-miR-221-3p	0.099403	0.60	0.27
hsa-miR-320a	0.109399	0.60	0.28
hsa-miR-339-3p	0.154338	0.58	0.29
hsa-miR-301a-3p	0.160690	0.57	0.30
hsa-miR-652-3p	0.160690	0.56	0.31
hsa-miR-30b-5p	0.160690	0.56	0.32
hsa-miR-25-3p	0.186436	0.57	0.33
Early HCC vs CLD
hsa-miR-21-5p	0.113812	0.64	0.20
hsa-miR-320d	0.302365	0.61	0.16
hsa-miR-652-3p	0.382238	0.60	0.23
hsa-miR-320a	0.432100	0.58	0.01
hsa-miR-423-5p	0.432100	0.56	0.06
hsa-miR-301a-3p	0.432100	0.57	0.09
hsa-miR-15b-5p	0.432100	0.57	0.07
hsa-miR-186-5p	0.432100	0.57	0.15
hsa-miR-221-3p	0.432100	0.56	0.24
hsa-miR-25-3p	0.432100	0.56	0.15
Early HCC vs cirrhosis
hsa-miR-652-3p	0.038792b	0.69	0.34
hsa-miR-221-3p	0.094025	0.66	0.29
hsa-miR-151a-5p	0.201979	0.63	0.15
hsa-miR-21-5p	0.201979	0.63	0.24
hsa-miR-15b-5p	0.217324	0.61	0.10
hsa-miR-25-3p	0.217324	0.61	0.15
hsa-miR-320a	0.306457	0.59	0.00
hsa-miR-423-5p	0.306457	0.59	0.07
hsa-miR-320d	0.306457	0.59	0.15
hsa-miR-28-5p	0.306457	0.59	0.20

^aQ-value < 0.001.

^bQ-value < 0.05.

AUC: Area under the curve; CLD: Chronic liver disease; HCC: Hepatocellular carcinoma; miRNA: MicroRNA; miR: MicroRNA.