Comprehensive evaluation of microRNA as a biomarker for the diagnosis of hepatocellular carcinoma

Table 1 Overview of the 26 selected known and putative microRNA candidates from next-generation sequencing and real-time quantitative PCR

Known miRNA	Fold change	AUC	P value (Wilcoxon)	Method	Comparison
hsa-miR-185-5p	2.01	0.77	0.000099c	NGS	All HCC vs CLD
hsa-miR-320a1	1.52	0.74	0.000335c
hsa-miR-423-5p	-1.22	0.69	0.004126b
hsa-miR-664a-5p	1.38	0.69	0.005695b
hsa-miR-203a	-2.73	0.67	0.01167a
hsa-miR-320d	2.56	0.66	0.017151a
hsa-miR-21-5p1	1.34	0.66	0.020486a
hsa-miR-28-5p	-1.65	0.80	0.0004c	RT-qPCR
hsa-miR-21-5p1	1.59	0.79	0.0006c
hsa-miR-103a-3p	-1.65	0.78	0.012a
hsa-miR-301a-3p	-1.50	0.78	0.013a
hsa-miR-30b-5p	-1.63	0.75	0.0033b
hsa-miR-495-3p	-1.50	0.73	0.0068b
hsa-miR-320a1	1.37	0.72	0.0093b
hsa-miR-30a-3p	-1.60	0.72	0.008145b	NGS	Early HCC vs CLD
hsa-miR-25-3p	1.75	0.71	0.01019a
hsa-miR-7706	1.67	0.69	0.02002a
hsa-miR-186-5p1	1.68	0.68	0.02364a
hsa-miR-15b-5p	4.21	0.97	< 0.0001c	RT-qPCR
hsa-miR-339-3p	-2.73	0.87	0.0006c
hsa-miR-10b-5p	2.81	0.86	0.0007c
hsa-miR-151a-5p	-2.25	0.85	0.0011b
hsa-miR-652-3p	-2.22	0.85	0.001b
hsa-miR-32-5p	2.03	0.85	0.001b
hsa-miR-221-3p	-2.96	0.83	0.0021b
hsa-miR-486-5p	2.43	0.82	0.0034b
hsa-miR-186-5p1	1.46	0.75	0.0215a
Putative miRNA	Fold change	AUC	P value (Wilcoxon)	Method	Comparison
put-miR-6	3.63	0.68	0.001956b	NGS	All HCC vs CLD
put-miR-99	1.83	0.6	0.188		Early HCC vs CLD

¹MicroRNAs detected in both next-generation sequencing and real-time quantitative PCR.

^aP < 0.05.

^bP < 0.01.

^cP < 0.001.

AUC: Area under the curve; CLD: Chronic liver disease; HCC: Hepatocellular carcinoma; miRNA: MicroRNA; NGS: Next-generation sequencing; RT-qPCR: Real-time quantitative PCR; miR: MicroRNA; put-miR: Putative microRNA.