N-linked glycoproteomic profiling in esophageal squamous cell carcinoma

Table 2 Summary of sialic N-linked glycoproteins identified in esophageal squamous cell carcinoma by 2-dimensional gel electrophoresis

Spot No.	Accession	Protein identity (Theoretical MW/pI)	Ratio	P value	Scores	Unique Peptides	N-glycosylation sites
3569	ENOA_HUMAN	Alpha-enolase (47.1/7.7)	2 (↑)	0.002	802.0 (M:802.0)	18	2 (17, 102)
3570	IGKC_HUMAN	Ig kappa chain C region (11.6/5.5)	2.4 (↑)	0.006	54.4 (M:54.4)	2	0
3612	TRFE_HUMAN	Serotransferrin (77/7)	1.5 (↑)	0.004	927.5 (M:927.5)	25	1 (630)
3687	KV305_HUMAN	Ig kappa chain V-III region WOL (11.7/9.5)	1.7 (↑)	0.009	125.4 (M:125.4)	2	0
3720	A1AT_HUMAN	Alpha-1-antitrypsin (46.7/5.3)	1.9 (↑)	0.002	49.3 (M:49.3)	2	3 (70, 107, 271)
4746	FIBB_HUMAN	Fibrinogen beta chain (55.9/9.3)	2.8 (↓)	0.004	817.3 (M:817.3)	17	0
4784	ACTB_HUMAN	Actin, cytoplasmic 1 (41.7/5.2)	3.4 (↓)	0.002	43.6 (M:43.6)	2	1 (12)
4948	HBB_HUMAN	Hemoglobin subunit beta (16/6.9)	1.8 (↓)	0.006	129.3 (M:129.3)	4	0
4967	ACTA_HUMAN	Actin, aortic smooth muscle (42/5.1)	1.7 (↓)	0.009	402.1 (M:402.1)	9	1 (14)
4985	FHR2_HUMAN	Complement factor H-related protein 2 (30.6/6)	2 (↓)	0.002	271.1 (M:271.1)	5	0
4998	1433Z_HUMAN	14-3-3 protein zeta/delta (27.7/4.6)	6.6 (↓)	0.009	62.1 (M:62.1)	2	2 (173, 224)
5010	FIBA_HUMAN	Fibrinogen alpha chain (94.9/5.6)	2.1 (↓)	0.002	83.3 (M:83.3)	3	3 (288, 419, 831)
5019	ACTH_HUMAN	Actin, gamma-enteric smooth muscle (41.8/5.2)	1.6 (↓)	0.009	92.7 (M:92.7)	3	1 (13)
5031	KV204_HUMAN	Ig kappa chain V-II region TEW (12.3/5.6)	1.5 (↓)	0.008	84.9 (M:84.9)	2	0
5057	CATB_HUMAN	Cathepsin B (37.8/5.9)	1.6 (↓)	0.009	84.8 (M:84.8)	2	2 (192, 289)
5069	FABP5_HUMAN	Fatty acid-binding protein, epidermal (15.2/7.5)	1.5 (↓)	0.005	88.7 (M:88.7)	2	0
5076	CRYAB_HUMAN	Alpha-crystallin B chain (20.1/6.9)	3.8 (↓)	0.009	240.1 (M:240.1)	6	0
5081	TAGL_HUMAN	Transgelin (22.6/9.4)	4.1 (↓)	0.009	134.7 (M:134.7)	3	0
5083	HSP71_HUMAN	Heat shock 70 kDa protein 1A/1B (70/5.4)	1.9 (↓)	0.004	105.8 (M:105.8)	3	1 (432)
5098	K1C17_HUMAN	Keratin, type I cytoskeletal 17 (48.1/4.8)	2.2 (↓)	0.002	198.2 (M:198.2)	5	0
5128	CYC_HUMAN	Cytochrome c (11.7/10.1)	2.6 (↓)	0.006	97.4 (M:97.4)	3	0
5164	S10A9_HUMAN	Protein S100-A9 (13.2/5.7)	1.7 (↓)	0.009	85.7 (M:85.7)	3	0
5173	S10A8_HUMAN	Protein S100-A8 (10.8/6.6)	1.5 (↓)	0.002	421.3 (M:421.3)	8	0