RING finger and WD repeat domain 3 regulates proliferation and metastasis through the Wnt/β-catenin signalling pathways in hepatocellular carcinoma

Figure 2 Validation of lentivirus knockdown efficiency in cell lines. A: RING finger and WD repeat domain 3 (RFWD3) expression in BEL-7404 and Hepatocellular carcinoma (HCC)-LM3 cells obtained using quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis; B and C: The shRNAi group shows obvious knockdown of RFWD3 [BEL-7404: qRT-PCR: ^bP < 0.01, western blot (WB): ^bP < 0.01; HCC-LM3: qRT-PCR: ^bP < 0.01, WB: ^bP < 0.01]; D: Confirmation of the lentivirus transduction efficiency using GFP fluorescence imaging.