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©The Author(s) 2022.
World J Gastroenterol. Jul 21, 2022; 28(27): 3422-3434
Published online Jul 21, 2022. doi: 10.3748/wjg.v28.i27.3422
Published online Jul 21, 2022. doi: 10.3748/wjg.v28.i27.3422
Figure 3 Confirmation of the expression of apoptosis-inducing factor followed by an increase in apoptosis in pancreatic cancer cells (AsPC-1 and CFPAC-1).
A: Immunocytochemical staining for apoptosis-inducing factor (AIF) release from mitochondria and translocation into the nucleus. Red dye: AIF; Green dye: Mitochondrial indicator. Scale bars: 10 μm; B: Quantitative analysis of the mean fluorescence intensity (MFI) of AIF in CFPAC-1; C: Quantitative analysis of the MFI of AIF in AsPC-1; D: Fluorescent microphotographs for colorimetric terminal deoxynucleotidyl transferase -mediated dUTP nick end labeling (TUNEL) detection. Green dye: TUNEL staining. Scale bars: 50 μm; E: Quantitative analysis of the % ratio of TUNEL detected cells per total pancreatic cancer cells; F: Representative flow cytometry plots using Annexin V-fluorescein-5-isothiocyanate/propidium iodide staining for apoptosis; G: Representative microphotographs to compare cell condition after calcium supply; H: Quantitative analysis of the percent cell viability following calcium supply. The cells were treated with 2.5 mM lactate calcium salt for 72 h. Results represent the mean ± SD. aP < 0.001 vs untreated. CaLac: Lactate calcium salt; AIF: Apoptosis-inducing factor; MFI: Mean fluorescence intensity; TUNEL: Transferase-mediated dUTP nick end labeling.
- Citation: Jeong KY, Sim JJ, Park M, Kim HM. Accumulation of poly (adenosine diphosphate-ribose) by sustained supply of calcium inducing mitochondrial stress in pancreatic cancer cells. World J Gastroenterol 2022; 28(27): 3422-3434
- URL: https://www.wjgnet.com/1007-9327/full/v28/i27/3422.htm
- DOI: https://dx.doi.org/10.3748/wjg.v28.i27.3422