Involvement of Met receptor pathway in aggressive behavior of colorectal cancer cells induced by parathyroid hormone-related peptide

Figure 7 Parathyroid hormone-related peptide attenuates the cytotoxicity of CPT11, oxaliplatin, and doxorubicin through the Met signaling pathway. A-C: HCT116 cells were pre-incubated with SU11274, a specific Met inhibitor, and then treated with PTHrP (10^-8 M) and/or irinotecan (CPT-11, 10 μM) (A), oxaliplatin (OXA, 10 μM) (B), or doxorubicin (DOXO, 5 μM) (C) for 24 h. The number of viable cells was quantified by Trypan blue technique. In each experiment, a control with DOXO, the vehicle of the inhibitor, was performed. Graph bars represent the average of the results obtained from two independent experiments. CPT-11: Irinotecan; DMSO: Dimethylsulfoxide; DOXO: Doxorubicin; OXA: Oxaliplatin; PTHrP: Parathyroid hormone-related peptide; SU: SU11274. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001.