Involvement of Met receptor pathway in aggressive behavior of colorectal cancer cells induced by parathyroid hormone-related peptide

Figure 4 Parathyroid hormone-related peptide increases mRNA levels of Met in HCT116 cells. Colon cancer cells were exposed to parathyroid hormone-related peptide (PTHrP) for 15 min, followed by real-time polymerase chain reaction analysis to detect Met mRNA levels as described in Materials and Methods to evaluate whether PTHrP modulates Met mRNA levels in HCT116 cells. Graph bars represent the average of the results obtained from three independent experiments. GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; Met: Receptor tyrosine kinase Met; PTHrP: Parathyroid hormone-related peptide. ^aP < 0.05.