Neutrophil extracellular traps participate in the development of cancer-associated thrombosis in patients with gastric cancer

Figure 7 Tumor-bearing mice show a greater ability to form thrombi by inferior vena cava flow restriction. A and B: The values for weight and length of thrombi present in control, tumor-bearing mice, DNase I infused tumor-bearing mice at 6 h after surgery. Each group, n = 9; C and D: Values for weight and length of thrombi present in mice at 48 h after surgery. Each group, n = 5; E and F: Confocal imaging of thrombi derived from control mice and tumor-bearing mice with Ly6G and citH3 staining. Magnification 10×; scale bars: 200 μm. Red-Ly6G, Green-citH3, and Blue-DAPI; G and H: Magnified (40×) part of thrombi derived from control mice and tumor-bearing mice. Scale bars: 50 μm. Red-Ly6G, Green-citH3, and Blue-DAPI; I and J: Fibrin formation levels in the plasma of control, tumor-bearing mice, or DNase-I-infused tumor-bearing mice were detected by turbidity measurement at 405 nm, and TAT complex levels were detected by ELISA. Each group, n = 8. All values are the mean ± SD. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001; ^dP < 0.0001. DNase I: Deoxyribonuclease I; TAT: Thrombin–antithrombin.