Neutrophil extracellular traps participate in the development of cancer-associated thrombosis in patients with gastric cancer

Figure 5 NETs promote platelet adhesion and prothrombotic state. A: Isolated platelets were incubated on glass slides which were coated with 1% dBSA, NETs (μg DNA/mL), or NETs pretreated with DNase I, APC, and sivelestat alone or together, followed by the F-actin components of platelets with 594-phalloidin staining. Magnification 63×; scale bars: 10 μm. Red-platelets; B: The percentage of area coverage of platelet adhesion was defined as the rate of red area in the total area and analyzed with ImageJ software; C: Isolated platelets were cocultured with different concentrations of NETs for 30 min with or without DNase I, APC and sivelestat treatment alone or together, and plasma fibrin formation was tested using turbidity measurements and monitored OD at 405 nm; D: TAT complex level of activated platelets was analyzed by ELISA. All values are the mean ± SD. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001; ^dP < 0.0001. dBSA: Denatured bovine serum albumin; NETs: Neutrophil extracellular traps; DNase I: Deoxyribonuclease I; APC: Activated protein C; TAT: Thrombin–antithrombin.