Neutrophil extracellular traps participate in the development of cancer-associated thrombosis in patients with gastric cancer

Figure 4 NETs contribute to hypercoagulation of platelets. A: PS exposure and P-selectin expression were measured when isolated platelets were cocultured with BETs (μg, DNA/mL) or in the presence of DNase I, activated protein C, and sivelestat alone or together by confocal microscopy. Magnification 63×; scale bars: 10 μm. Red-platelets, Green-Lactadherin, and Blue-P-selectin; B and C: PS exposure and P-selectin expression are indicated as MFI. MFI was defined as the ratio of total fluorescence intensity to the area; D and E: The rates of PS-positive platelets and P-selectin-positive platelets were detected by flow cytometry. All values are the mean ± SD. ^cP < 0.001; ^dP < 0.0001. PS: Phosphatidylserine; GC: Gastric cancer; DNase I: Deoxyribonuclease I; APC: Activated protein C; MFI: Mean fluorescence intensity; Lact: Lactadherin; CD41: Platelet; NET: Neutrophil extracellular trap.