Upregulated adenosine 2A receptor accelerates post-infectious irritable bowel syndrome by promoting CD4+ T cells’ T helper 17 polarization

Figure 3 Adenosine 2A receptor expression level in CD4+ T cells isolated and purified from the post-infectious irritable bowel syndrome mouse. A: Fluorescence-activated cell sorting results showed that CD4+ T cells (P3) were successfully isolated and purified; B-C: Immunohistochemistry staining of adenosine 2A receptor (A2AR) in CD4+ T cells; D: Western blot assay evaluated A2AR expression levels in CD4+ T cells isolated from different groups; E: Relative A2AR mRNA levels in the three groups. ^aP < 0.05. A2AR: Adenosine 2A receptor; PI-IBS: Post-infectious irritable bowel syndrome; NS: Not significant.