Mechanism and therapeutic strategy of hepatic TM6SF2-deficient non-alcoholic fatty liver diseases via in vivo and in vitro experiments

Figure 5 The activity of fatty acid synthesis was enhanced in TM6SF2 knockdown group. A: Pathway enrichment of the up-regulated differentially expressed genes by Kyoto Encyclopedia of Genes and Genomes analysis; B: Microarray heatmap of genes involved in fatty acid metabolism in TM6SF2-knockdown cells; C: The SREBP-1c target protein levels in L02 and HepG2 cells after palmitic acid (PA) treatment; D: The SREBP-1c target protein levels in TM6SF2-knockdown cells after PA treatment; E: Immunoblot analysis of SREBP-1c in cytoplasmic and nuclear extracts; F: Hepatic malonyl-CoA contents of mice in two groups (n = 5 mice per group); G: The mRNA levels of genes involved in fatty acid synthesis (Srebf1, Fasn, Scd1, Acaca and Hmgcr) and uptake (Cd36, Slc27a1 and Fabp1). ^aP < 0.05; ^bP < 0.01; ^cP < 0.001. HFD: High-fat diet; PA: Palmitic acid; ACC: Acetyl-CoA carboxylase; μM: μmol/L.