Fecal gene detection based on next generation sequencing for colorectal cancer diagnosis

Table 9 Analysis of pathogenic mutation sites in tumor tissues

Gene	Location	Amino acid mutation	Mutation frequency	N	M
TP53	Exon 4	R110L	1
	Exon 5	R175H	4
		Y163C	1
		C176Y	1
	Exon 6	R1961	2
		Y220C	2
	Exon 7	R248Q/W	3
		G245S/C	2
	Exon 8	R273H/C	4
		R282W	2
		R3061	1
	Exon 10	R3421	1	24/40	23/31
KRAS	Exon 2	G12D/V/S	22
		G13D/C	4
	Exon 3	Q61H	1	27/30	27/29
APC	Exon 17	Q8861	1
		S1483fs	1
		R8761	2
		R14501	2
		E13061	1
		Q12941	1
		G13121	1	9/15	9/12

¹Indicates stop codon.

N: Pathogenic sites/total mutation sites; M: Number of pathogenic cases/total number of mutation cases.