Fecal gene detection based on next generation sequencing for colorectal cancer diagnosis

Table 13 "Undetected" gene mutation sites in preoperative feces

Gene	Location	Amino acid mutation	Mutation start position	Mutation end position	Number of cases	Undetected sites/total mutation sites
TP53	Exon 4	A84G	7579436	7579436	1
	Exon 5	S166P	7578434	7578434	1
	Exon 7	N247D	7577542	7577542	3
	Exon 8	L289P	7577072	7577072	1	6/27
CDKN2A	Exon 2	V51A	21971206	21971206	2
		L63P	21971170	21971170	1	3/4
HRAS	Exon 2	G12R	534289	534289	1	1/10
EGFR	Exon 20	I821T	55249164	55249164	1	1/2
IDH1	Exon 4	R119Q	209113151	209113151	1	1/2
PDGFRA	Exon 13	G594fs	55141059	55141059	1	1/8

fs: Frameshift mutation.