Fecal gene detection based on next generation sequencing for colorectal cancer diagnosis

Table 10 Analysis of pathogenic mutation sites in preoperative feces

Gene	Location	Amino acid mutation	Mutation frequency	N	M
TP53	Exon 5	C176Y	1
		F134V	1
	Exon 6	R1961	1
	Exon 7	N235D	1
		R248Q	3
		G245C/S	2
	Exon 8	R273H/C	3	12/27	10/16
KRAS	Exon 2	G12D/V	5
		G13D	1
	Exon 3	Q61H	1
		A59T	1	8/11	7/10
APC	Exon 17	R14501	1
		Q8861	1
		Q12941	1	3/3	3/3

¹Indicates stop codon.

N: Pathogenic sites/total mutation sites; M: Number of pathogenic cases/total number of mutation cases.