Downregulation of TNFR2 decreases survival gene expression, promotes apoptosis and affects the cell cycle of gastric cancer cells

Figure 5 Apoptosis distribution analysis in nonsilenced AGS cells, cells with TNFR1 downregulated (shTNFR1), and cells with TNFR2 downregulated (shTNFR2). A: Cells without additional treatment (control); B: Cells treated with the Helicobacter pylori extract; A and B: Representative images of the cell distribution and percentage in the different quadrants indicating viable cells (Annexin V-/PI-) in the lower left, early apoptotic cells (Annexin V+/PI-) in the lower right and late apoptotic cells (Annexin V+/PI+) in the upper right; C and D: Histogram bars represent the mean ± SD from three independent trials for early (C) and late (D) apoptosis. Statistically significant difference: ^aP < 0.05; ^bP < 0.01. eHP: Helicobacter pylori extract.