Downregulation of TNFR2 decreases survival gene expression, promotes apoptosis and affects the cell cycle of gastric cancer cells

Figure 2 Relative expression of tumour necrosis factor-α signalling pathway genes. Expression levels of TNFA, TNFR1, TNFR2, TRADD, TRAF2, CFLIP, CASP8, CASP3, NFKB1 and NFKB2 mRNA in nonsilenced AGS, AGS with TNFR1 downregulation (shTNFR1), and AGS with TNFR2 downregulation (shTNFR2) either not treated (control) or treated with a Helicobacter pylori extract. A: TNFA; B: TNFR1; C: TNFR2; D: TRADD; E: TRAF2; F: CFLIP; G: CASP8; H: CASP3; I: NFKB1; J: NFKB2. Bars represent the mean ± SD from three independent trials. The dotted line indicates relative quantification: RQ = 1.0. Statistically significant difference: ^aP < 0.05; ^bP < 0.01; ^cP < 0.001. eHP: Helicobacter pylori extract.