Novel multiplex stool-based assay for the detection of early-stage colon cancer in a Chinese population

doi:10.3748/wjg.v28.i24.2705

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Jun 28, 2022 (publication date) through Feb 21, 2025

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Clinical and Translational Research

World J Gastroenterol. Jun 28, 2022; 28(24): 2705-2732
Published online Jun 28, 2022. doi: 10.3748/wjg.v28.i24.2705

Table 1 Primers for pyrosequencing of three DNA methylation biomarkers

Target gene	Primer	Sequence	Size (bp)	Target DMS
PAX8	Forward	5’-GGGGGTTAGGGGATTTTGATTATA-3’	166	chr2:114035984; 114035988; 114035995; 114035998; 114036006
	Reverse	Biotin-5’-ATCTCATACCCTTCTCCTAAATTTATAC-3’
	Sequencing	5’-ATGGAGTTGTGAGGT-3’
RASSF1	Forward	5’-TTTATTTATTGGGTGGGGTAGGA-3’	141	chr3:50378714; 50378718
	Reverse	Biotin-5’-CCTCAAAATCACCATCCAACCTCTAC-3’
	Sequencing	5’-GGGAGATAGGTTAGTAGTTTTA-3’
SFRP2	Forward	5’-GATTAGGGATAATTAGGTAAAAGGAGTT-3’	166	chr4:154711281; 154711305
	Reverse	Biotin-5’-ATTCATCCCCTACCTACCAAAAAACACC-3’
	Sequencing	5’-AGTTAGAGATATTAGATTTTAGG-3’

PAX8: Paired box 8; RASSF1: Ras-association domain family 1; SFRP2: Secreted frizzled-related protein 2; DMS: Differentially methylated site.

Citation: Jiang HH, Xing SW, Tang X, Chen Y, Lin K, He LW, Lin MB, Tang EJ. Novel multiplex stool-based assay for the detection of early-stage colon cancer in a Chinese population. World J Gastroenterol 2022; 28(24): 2705-2732
URL: https://www.wjgnet.com/1007-9327/full/v28/i24/2705.htm
DOI: https://dx.doi.org/10.3748/wjg.v28.i24.2705