Family with sequence similarity 134 member B-mediated reticulophagy ameliorates hepatocyte apoptosis induced by dithiothreitol

Figure 5 Impact of dithiothreitol treatment on apoptosis of buffalo rat liver 3A cells lacking FAM134B. A and B: Buffalo rat liver 3A (BRL-3A) cells were transfected with FAM134B small interfering RNAs (siRNAs) 1, 2, and 3; immunoblot was used to detect the transfection efficiency of siRNA. Protein levels were normalized to β-actin; C and D: BRL-3A cells were transfected with FAM134B siRNA, followed by treatment with 2.0 mmol/L dithiothreitol (DTT) for 24 h. Immunoblot was used to detect the expression of FAM134B in BRL-3A cells. Protein levels were normalized to β-actin; E and F: BRL-3A cells were transfected with FAM134B siRNA, followed by treatment with 2.0 mmol/L DTT for 24 h. Immunoblot showed the expression of cleaved caspase-3 and cyt c. Protein levels were normalized to β-actin; G and H: BRL-3A cells were transfected with FAM134B siRNA, followed by treatment with 2.0 mmol/L DTT for 24 h. Representative results showed the apoptotic rate in BRL-3A cells. Untransfected cells served as controls. Cells transfected with control siRNA served as transfection controls. The lower right quadrant represents the early apoptotic cells and the upper right quadrant represents the late apoptotic cells. Values are mean ± SD (n = 3), ^aP < 0.05 vs control group; ^bP < 0.05 vs transfection control siRNA group.