Family with sequence similarity 134 member B-mediated reticulophagy ameliorates hepatocyte apoptosis induced by dithiothreitol

Figure 2 Impact of dithiothreitol treatment on the formation of autolysosomes in buffalo rat liver 3A cells. After dithiothreitol treatment for 0, 3, 6, 12, 24, and 48 h, the buffalo rat liver 3A cells labeled with endoplasmic reticulum (ER)-Tracker Red and Lyso-Tracker Green were observed and captured under confocal fluorescence microscopy (200 ×) in a live cell imaging experiment. Insets show the magnification of the pictures. Scale bars indicate 100 μm. Arrows head to indicate ER-localized lysosomes. Values are represented as mean ± SD (n = 3), ^aP < 0.05 vs 0 h group; ^bP < 0.05 vs 48 h group.