Family with sequence similarity 134 member B-mediated reticulophagy ameliorates hepatocyte apoptosis induced by dithiothreitol

Figure 1 Impact of the endoplasmic reticulum stressor, dithiothreitol, on endoplasmic reticulophagy mediated by family with sequence similarity 134 member B in buffalo rat liver 3A cells. A and B: Buffalo rat liver 3A (BRL-3A) cells were treated with 2.0 mmol/L dithiothreitol (DTT) for the time intervals (0, 3, 6, 12, 24, 48 h); Western blot showed the effect of endoplasmic reticulum (ER) stressor, DTT, on expression of the ER stress-related proteins glucose-regulated protein 78 (GRP78), calnexin (CNX), and C/EBP homologous protein (CHOP); β-actin was used as a control for normalization; C and D: Analysis of autophagy related gene 12 (ATG12), family with sequence similarity 134 member B (FAM134B), and microtubule-associated protein 1 light chain 3 (LC3) protein expression by western blot. Protein levels were normalized to β-actin; E: BRL-3A cells were treated with 2.0 mmol/L DTT for 0 and 24 h; co-immunoprecipitation analysis detected the presence of CNX-FAM134B-LC3 complex in BRL-3A cells. Values are represented as mean ± SD (n = 3), ^aP < 0.05 vs 0 h group; ^bP < 0.05 vs 48 h group.