Impact of radiotherapy on the immune landscape in oesophageal adenocarcinoma

Figure 3 Oesophageal adenocarcinoma patients were screened for the surface expression of immune checkpoints ex vivo by flow cytometric analysis. Subcohorts where ionising radiation induced upregulation and downregulation of immune checkpoints (ICs). Inhibitory ICs are expressed at a higher level with conventional and hypofractionated dosing regimens in one cohort (n = 8). Inhibitory ICs are expressed at a lower level with conventional and hypofractionated dosing regimens in a separate cohort (n = 9). A and B: Increasing and decreasing cohort of PD-1; C and D: Increasing and decreasing cohort of PD-L1; E and F: Increasing and decreasing cohort of TIGIT; G and H: Increasing and decreasing cohort of TIM-3. ^aP < 0.05; ^bP < 0.01 by Wilcoxon signed rank test.