Serotonin type 3 receptor subunit gene polymorphisms associated with psychosomatic symptoms in irritable bowel syndrome: A multicenter retrospective study

Table 3 Differences between single-nucleotide polymorphisms in depressive and anxiety symptoms, separately analyzed with ANOVA using the dominant model and the recessive model

Models	Mental symptoms	SNPs	Total	Sex		IBS subtypes
				Male	Female	IBS-C	IBS-D	IBS-M
Dominant model	Depressive symptoms	HTR3A c.-42C>T (rs1062613)	2.541	4.149 ↑	0286	0.998	0.376	0.694
		HTR3B c.386A>C (rs1176744)	0.067	0.005	0.198	0.282	0.124	0.232
		HTR3C c.489C>A (rs6766410)	7.475 ↑	0.672	7.040 ↑	0.261	5.670 ↑	2.328
		HTR3E c.*76G>A (rs62625044)	0.054	0.013	0.180	0.208	0.461	0.010
	Anxiety symptoms	HTR3A c.-42C>T (rs1062613)	0.511	0.210	0.280	0.604	0.672	0.162
		HTR3B c.386A>C (rs1176744)	0.926	0.406	0.801	0.468	1.369	0.770
		HTR3C c.489C>A (rs6766410)	6.535 ↑	0.158	7.550 ↑	0.186	13.444 ↑	0.045
		HTR3E c.*76G>A (rs62625044)	1.055	0.429	0.853	0.000	0.495	1.109
	Somatization symptoms	HTR3A c.-42C>T (rs1062613)	0.016	0.141	0.106	0.086	0.017	0.203
		HTR3B c.386A>C (rs1176744)	0.217	0.332	0.326	0.407	0.415	2.125
		HTR3C c.489C>A (rs6766410)	0.784	0.035	1.085	0.269	0.000	1.962
		HTR3E c.*76G>A (rs62625044)	0.002	1.554	0.263	0.128	0.729	0.354
Recessive model	Depressive symptoms	HTR3A c.-42C>T (rs1062613)	0.002	0.421	0.132	0.093	1.320	1.064
		HTR3B c.386A>C (rs1176744)	1.118	2.027	0.107	1.741	0.197	0.744
		HTR3C c.489C>A (rs6766410)	0.047	0.821	0.401	2.306	6.190 ↑	0.676
		HTR3E c.*76G>A (rs62625044)	0.677	-	0.574	0.541	-	-
	Anxiety symptoms	HTR3A c.-42C>T (rs1062613)	0.479	0.027	0.549	0.057	0.315	0.350
		HTR3B c.386A>C (rs1176744)	0.872	0.081	1.691	0.476	0.361	1.923
		HTR3C c.489C>A (rs6766410)	0.028	2.562	0.344	0.570	3.509	2.093
		HTR3E c.*76G>A (rs62625044)	0.216	-	0.179	0.242	-	-
	Somatization symptoms	HTR3A c.-42C>T (rs1062613)	0.217	0.018	0.257	0.138	0.092	0.431
		HTR3B c.386A>C (rs1176744)	6.482 ↑	2.245	3.575	0.142	14.033 ↑	0.341
		HTR3C c.489C>A (rs6766410)	0.468	1.813	0.128	4.715 ↓	0.002	0.113
		HTR3E c.*76G>A (rs62625044)	0.001	-	0.011	0.000	-	-

F values are shown in the table. Arrows represent the direction of the associations. ↑ dependent variable increases from major to minor alleles; ↓ dependent variable decreases from major to minor alleles. The underlined values were significant. Values in bold were “nominally significant” (i.e., lacking significance after false discovery rate multiple testing correction). IBS: Irritable bowel syndrome; IBS-C: Constipation-predominant IBS; IBS-D: Diarrhea-predominant IBS; IBS-M: IBS with mixed bowel habits; SNPs: Single-nucleotide polymorphisms.