Long noncoding RNA TNFRSF10A-AS1 promotes colorectal cancer through upregulation of HuR

Figure 3 miR-3121-3p is downregulated in colorectal cancer. A: We used three websites (miRDB, DIANA and LncRNAMAP) to predict 15 microRNAs with binding sites for TNFRSF10A-AS1; B: miR-3121-3p expression was increased in cells transfected with si-lnc and decreased in cells transfected with oe-lnc; C: miR-3121-3p was downregulated in colorectal cancer tumor tissues compared to matched nontumor tissues (n = 40); D: Levels of miR-3121-3p in colon cancer cell lines were detected by quantitative real-time polymerase chain reaction; E: Schematic illustration of the predicted binding sites of miR-3121-3p in the TNFRSF10A-AS1 sequence; F: Relative luciferase activities after cotransfection with TNFRSF10A-AS1-WT, TNFRSF10A-AS1-MUT, miR-3121-3p mimics or NC in DLD1 cells. WT: Wild-type; MUT: Mutant.