Long noncoding RNA TNFRSF10A-AS1 promotes colorectal cancer through upregulation of HuR

Figure 2 TNFRSF10A-AS1 promotes colon cancer cell proliferation, migration and invasion as well as inhibits cell apoptosis in vitro. A and B: Downregulation of TNFRSF10A-AS1 decreased cell viability and clonogenicity in DLD1 and HT29 cells, whereas upregulation of TNFRSF10A-AS1 greatly increased cell viability and clonogenicity in HCT116 and SW480 cells; C: Silencing TNFRSF10A-AS1 arrested cells at the G1/S transition, whereas overexpressing TNFRSF10A-AS1 facilitated this transition; D: Downregulation of TNFRSF10A-AS1 enhanced cell apoptosis, whereas upregulation of TNFRSF10A-AS1 inhibited cell apoptosis; E: Western blot analysis showed that TNFRSF10A-AS1 knockdown decreased the expression of cyclin D1 and PCNA but increased the activation of caspase-3 and PARP, whereas TNFRSF10A-AS1 overexpression had the opposite effect; F and G: Silencing TNFRSF10A-AS1 suppressed DLD1 and HT29 cell migration and invasion abilities, whereas these abilities were significantly enhanced by overexpressing TNFRSF10A-AS1 in HCT116 and SW480 cells.